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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: CENPF All Species: 9.39
Human Site: S1818 Identified Species: 17.22
UniProt: P49454 Number Species: 12
    Phosphosite Substitution
    Charge Score: -0.17
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P49454 NP_057427.3 3210 367764 S1818 V K P T G E C S G E Q S P D T
Chimpanzee Pan troglodytes XP_001171549 3114 357698 S1722 V K P T G E C S G E Q S P D T
Rhesus Macaque Macaca mulatta XP_001105956 3113 357422 S1721 V K L T G E C S G E Q S Q D T
Dog Lupus familis XP_852813 1449 166096 G222 E Q Q N E Q K G A S L N P L D
Cat Felis silvestris
Mouse Mus musculus
Rat Rattus norvegicus
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001510329 2965 340022 N1691 E D T T L A E N E S S G L G D
Chicken Gallus gallus O42184 1433 161009 G206 V S N L S E A G S L K K G E R
Frog Xenopus laevis P85120 2058 236320 K831 Q E M V Q L E K D N K I L Q K
Zebra Danio Brachydanio rerio XP_002665261 2708 311087 K1481 E D M S H V L K E T V R T M E
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q9VJE5 1690 189046 K463 D L E S K I T K L V S A T P S
Honey Bee Apis mellifera XP_001120388 2064 240016 C837 M K D A L G K C V D E I E K L
Nematode Worm Caenorhab. elegans P02566 1966 225108 E739 Y A I L A A K E A K S D D D K
Sea Urchin Strong. purpuratus XP_796801 3636 416057 M2077 L Q D S N D K M I K L Q A D M
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P25386 1790 206434 T563 Q T I S E L L T T S L T A A D
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 95.8 91.4 32.8 N.A. N.A. N.A. N.A. 50.5 20.4 21.5 31.3 N.A. 21.3 22.9 20.1 22
Protein Similarity: 100 96.4 93.8 39.1 N.A. N.A. N.A. N.A. 67.5 32.4 38.6 51.2 N.A. 35.2 41.3 36.4 43.7
P-Site Identity: 100 100 86.6 6.6 N.A. N.A. N.A. N.A. 6.6 13.3 0 0 N.A. 0 6.6 6.6 6.6
P-Site Similarity: 100 100 86.6 26.6 N.A. N.A. N.A. N.A. 13.3 26.6 13.3 6.6 N.A. 20 26.6 13.3 40
Percent
Protein Identity: N.A. N.A. N.A. N.A. 21.7 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 36.5 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 0 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 8 0 8 8 16 8 0 16 0 0 8 16 8 0 % A
% Cys: 0 0 0 0 0 0 24 8 0 0 0 0 0 0 0 % C
% Asp: 8 16 16 0 0 8 0 0 8 8 0 8 8 39 24 % D
% Glu: 24 8 8 0 16 31 16 8 16 24 8 0 8 8 8 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 24 8 0 16 24 0 0 8 8 8 0 % G
% His: 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 16 0 0 8 0 0 8 0 0 16 0 0 0 % I
% Lys: 0 31 0 0 8 0 31 24 0 16 16 8 0 8 16 % K
% Leu: 8 8 8 16 16 16 16 0 8 8 24 0 16 8 8 % L
% Met: 8 0 16 0 0 0 0 8 0 0 0 0 0 8 8 % M
% Asn: 0 0 8 8 8 0 0 8 0 8 0 8 0 0 0 % N
% Pro: 0 0 16 0 0 0 0 0 0 0 0 0 24 8 0 % P
% Gln: 16 16 8 0 8 8 0 0 0 0 24 8 8 8 0 % Q
% Arg: 0 0 0 0 0 0 0 0 0 0 0 8 0 0 8 % R
% Ser: 0 8 0 31 8 0 0 24 8 24 24 24 0 0 8 % S
% Thr: 0 8 8 31 0 0 8 8 8 8 0 8 16 0 24 % T
% Val: 31 0 0 8 0 8 0 0 8 8 8 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _